The Problem
A Thermo NanoDrop 2000c spectrophotometer was failing during startup and could not complete wavelength verification. The failure prevented the instrument from being released for normal sample measurement and created uncertainty about the optical path.
Diagnostics
ScientifiCo inspected the startup sequence and focused on the components required for wavelength verification. The optical switch attempted to proceed, but the Xenon light source was not illuminating. Power and signal behavior at the lamp were checked, and no arc discharge or usable lamp output was observed.
The optical switch itself was confirmed to be functional, but it could not complete verification without a working source. The inspection confirmed an electrical failure of the Xenon lamp assembly.
Repair
The failed Xenon source was replaced with an Excelitas LS-288 lamp. Care was taken to restore the light source without disturbing the optical alignment more than necessary, because NanoDrop performance depends on stable lamp output and proper wavelength response.
Calibration
After lamp replacement, the system underwent optical calibration and wavelength verification using calibration check fluid. This step confirmed that the new light source was producing usable output and that the instrument could verify wavelength accuracy through its normal startup and calibration routines.
Result
The NanoDrop 2000c passed wavelength verification after service and returned to normal operating condition. The repair restored the instrument for routine UV-Vis microvolume measurements.
