Agilent HPLC Retention Time Shift Resolved by Clearing Pump Blockage
Retention time shift on an Agilent HPLC system was reported during routine runs, affecting method consistency and data reliability. Initial flushing revealed abnormally high back pressure even with the purge valve open, pointing to a restriction within the pump. Diagnostics confirmed a clogged PTFE frit inside the pump as the root cause of the pressure issue. After replacing the frit, system pressure returned to normal levels. A follow-up leak test initially failed, which led to further inspecti...
